GOAnno

GOAnno is a web tool that automatically annotates proteins according to the Gene Ontology using hierarchised multiple alignments. Positioning the query protein in its aligned functional subfamily represents a key step to obtain highly reliable predicted GO annotation based on the GOAnno algorithm.
1- Input your protein sequence
Please Enter or "cut and paste" your protein sequence here (in FASTA format).
It is advised to write your SWISS-PROT (ex : FGF2_HUMAN) or TrEMBL (ex : Q7KZ72) query protein accession (or ID.) directly after the greater-than (">") symbol of the description. If you do not, the results of the IPO (Initial Protein Ontology) will be empty.

or Enter your session ID (Results will be available during 1 week).

2- or
Options- Change default PipeAlign or GPO parameters
PipeAlign parameters
Expect-value cutoff for BlastP :
Maximum number of database sequences to show for BLASTP matches :
Low Complexity filter for BlastP : ON
Maximum number of protein sequences to show for MACS (Multiple Alignment of Complete Sequences):
GPO (Global Protein gene Ontology) parameters (GPO = IPO + PPO + MSO)
Percent identity cutoff to consider two proteins as homologs for PPO (Proximal Protein gene Ontology) :
f cutoff : p cutoff : for MSO (Mean Subfamily gene Ontology).
Help and Supplementary information